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        當前位置:首頁 >產品中心>細胞庫>人腫瘤細胞、癌細胞>HTB-55Calu-3 人類肺腺癌細胞(胸水)

        Calu-3 人類肺腺癌細胞(胸水)

        簡要描述:Calu-3 人類肺腺癌細胞(胸水)
        上海復祥生物提供各類細胞,代理美國ATCC品牌。

        • 產品型號:HTB-55
        • 廠商性質:生產廠家
        • 更新時間:2025-04-27
        • 訪  問  量:2696

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        Calu-3 人類肺腺癌細胞(胸水)

        ATCC® Number:HTB-55™    

        Designations:Calu-3

        Depositors:J Fogh

        Biosafety Level:1

        Shipped:frozen

        Medium & Serum:See Propagation

        Growth Properties:adherent

        Organism:Homo sapiens (human)

        Morphology:epithelial


        Source:Organ: lung
        Disease: adenocarcinoma
        Derived from metastatic site: pleural effusion


        Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
        Calu-3 人類肺腺癌細胞(胸水)

        Restrictions:The cells are distributed for research purposes only. The Memorial Sloan-Kettering Cancer Center releases the line subject to the following: 1.) The cells or their products must not be distributed to third parties. Commercial interests are the exclusive property of Memorial Sloan-Kettering Cancer Center. 2.) Any proposed commercial use of these cells must first be negotiated with The Director, Office of Industrial Affairs, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10021; phone (212) 639-6181; (212) 717-3439.

        Applications:transfection host (Roche FuGENE® Transfection Reagents)

        Tumorigenic:Yes

        Antigen Expression:Blood Type A; Rh+

        DNA Profile (STR):Amelogenin: X

        CSF1PO: 11,12

        D13S317: 12

        D16S539: 12,14

        D5S818: 11

        D7S820: 10,11

        THO1: 6,9.3

        TPOX: 8

        vWA: 16,17



        Cytogenetic Analysis:The stemline chromosome number is hypotriploid with the 2S component occurring at 1.4%. Approximay 20 markers were common to most S metaphases, of which i (1p), t(12;?) and t(18;?) were generally paired; t(6t?) had an HSR segment of q arm, and M7 had two secondary constrictions. Normal chromosomes 1, 13, 15 and 17 were absent, and the X was disomic. No Y chromosome was detected in the QM stained preparations.

        Isoenzymes:AK-1, 1

        ES-D, 1

        G6PD, B

        GLO-I, 1-2

        Me-2, 1

        PGM1, 1

        PGM3, 1-2



        Age:25 years

        Gender:male

        Ethnicity:Caucasian

        Comments:The patient had received prior therapy with cytoxan, bleomycin and adriamycin.

        Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
        Atmosphere: air, 95%; carbon dioxide (CO2), 5%
        Temperature: 37.0°C


        Subculturing:Protocol:
        1. Remove and discard culture medium.

        2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

        3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

          Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

        4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

        5. Add appropriate aliquots of the cell suspension to new culture vessels.

        6. Incubate cultures at 37°C.

        Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:6 is recommended
        Medium Renewal: 2 to 3 times per week




        MLE-12 小鼠肺上皮細胞

        saos-2 人成骨肉瘤細胞

        MA104 非洲綠猴胚胎腎上皮細胞

        ball-1 細胞

        nci-h226 人肺鱗癌細胞

        MCA38 小鼠結腸癌細胞

        LTPA 小鼠胰腺癌細胞

        NCI-H23 人非小細胞肺癌細胞

        293.00     人胚腎細胞

        293FT    人胚腎細胞-用于慢病毒包裝

        293TN    人胚腎細胞--用于慢病毒包裝

        293AV    人胚腎細胞-用于腺病毒包裝

        293T    人胚腎細胞

        208F    大鼠成纖維細胞

        786-O    人腎透明細胞腺癌細胞

        A172    人腦膠質母細胞瘤細胞

        A549    人肺癌細胞 細胞系購買

        AGS    人胃癌細胞

        BEL7402 人肝癌細胞系

        AZ-521    人胃癌細胞

        B16F0    小鼠黑色素瘤細胞

        Bel-7402    人肝癌細胞

        Bel-7404    人肝癌細胞

        BGC-823    人胃癌細胞

        C6    大鼠腦膠質瘤細胞

        SW480    人結腸腺癌細胞

        CaCo-2    人結腸癌細胞

        Cho-K1    倉鼠卵巢細胞亞株

        COS-7    非洲綠猴SV40轉化的腎細胞

        GES-1    人胃上皮細胞

        HCT-8    人回盲腸癌細胞

        HeLa

        Hepa1-6    小鼠肝癌細胞

        HepG2    人肝癌細胞

        hFOB1.19    人永生化成骨細胞

        HGC-27    人胃癌細胞

        Hs 746T    人胃癌細胞

        HT-29    人結腸癌細胞

        KB    人口腔表皮樣癌細胞

        LoVo    人結腸癌細胞

        LLC-PK1    豬腎近曲小管上皮細胞

        MCF-10A    人正常乳腺上皮細胞

        MCF-7    人乳腺癌細胞

        MDA-MB-231    人乳腺癌細胞

        MGC80-3    人胃癌細胞

        MKN-45    人胃癌細胞

        NCI-H460    人大細胞肺癌細胞

        NIH-3T3    小鼠成纖維細胞

        NUGC-3    人胃癌細胞

        Raw264-7    小鼠單核細胞白血病細胞

        SGC-7901    人胃癌細胞

        SMMC-7721    人肝癌細胞

        SNU-638    人胃癌細胞

        SNU-739    人肝癌細胞

        Tca8113    人舌鱗癌細胞    細胞系購買

        U251    人腦膠質瘤細胞

        U-373MG    人腦神經膠質瘤細胞

        U-87 MG    人腦星形膠質母細胞瘤

        BT-474    人乳腺導管癌細胞

        PANC-1    人胰腺癌細胞

        HEK293    人胚腎細胞

        Hep3B    人肝癌細胞

        HL-7702    人正常肝細胞

        Raw-Blue    小鼠單核細胞白血病細胞

        WEHI-231    小鼠B淋巴細胞

        OPM-2    人骨髓瘤細胞

        NCI-H929    人骨髓瘤細胞

        U266    人骨髓瘤細胞

        EJ    人膀胱癌細胞

        HOC    大鼠肝卵圓細胞

        RWPE-1    人正常前列腺上皮細胞

        CAL 27    人舌鱗癌細胞

        Calu-3    人肺腺癌細胞

        Eca-109    人食管癌細胞

        DLD-1    人結直腸腺癌上皮細胞

        5637.00     人膀胱癌細胞

        SK-OV-3    人卵巢癌細胞

        GBC-SD    人膽囊癌細胞

        BEL7402 人肝癌細胞系

        SW620    人結腸癌細胞

        MRC-5    人胚肺成纖維細胞     細胞系購買

        PC9    人肺癌細胞

        PC14    人肺癌細胞

        BxPC    人原位胰腺腺癌細胞

        PK(15)    豬腎上皮細胞

        MDCK    狗腎上皮細胞

        3D4/21    豬肺泡巨噬細胞

        MHCC-97L    人肝癌細胞(低轉移)

        MHCC-97H    人肝癌細胞系(高轉移)

        MiaCaPa-2    人胰腺癌細胞

        HN13    口腔鱗狀細胞癌細胞

        Bend3    小鼠腦微血管內皮細胞

        QBC939    人膽管癌細胞

        Ishikawa    人子宮內膜癌細胞

        A498    人腎癌細胞

        143B

        WEHI-231    小鼠B淋巴細胞

        H322    人非小細胞肺癌細胞

        Panc O2

        S8    BEL7402 人肝癌細胞系

        R5    細胞系購買

        Pig MSC    豬骨髓間質干細胞

        HCAEC    人冠狀動脈內皮細胞

        marc-145    猴胚胎腎上皮細胞

        3D4/21    豬肺泡巨噬細胞

        PK(15)    豬腎上皮細胞

        MADB-106    大鼠乳腺癌細胞

        Sf9    昆蟲卵巢細胞

        DADY

        HUVEC[HUV-EC-C]    人臍靜脈內皮細胞

        PC14    人肺癌細胞

        PC9    人肺癌細胞

        Eca-109    人食管癌細胞

        CAL 27    人舌鱗癌細胞

        HOC    肝卵圓細胞

        EJ    人膀胱癌細胞

        U266    人骨髓瘤細胞

        293-mTLR5    人胚腎細胞

        Raw-Blue    小鼠單核細胞白血病細胞

        HL-7702    人正常肝細胞

        Hep3B    人肝癌細胞

        U-373MG    人腦神經膠質瘤細胞

        Tca-8113    人舌鱗癌細胞

        SNU-739    人肝癌細胞

        SNU-638    人胃癌細胞

        NUGC-3    人胃癌細胞

        NIH-3T3    小鼠成纖維細胞

        MKN-45    人胃癌細胞

        LLC-PK1    豬腎近曲小管上皮細胞

        Hs 746T    人胃癌細胞

        Hepa1-6    小鼠肝癌細胞

        HCT-8    人回盲腸癌細胞

        BEL7402 人肝癌細胞系

        HaCaT    人永生化表皮細胞

        GES-1    人胃上皮細胞

        B16-F0    小鼠黑色素瘤細胞

        AZ-521    人胃癌細胞

        786-O    人腎透明細胞腺癌細胞

        208F    大鼠成纖維細胞

        293TN    人胚腎細胞--用于慢病毒包裝

        293FT    人胚腎細胞-用于慢病毒包裝

        GP-293    人胚腎細胞

        293-L.P.    人胚腎細胞

        HTB-55 Calu-3 人類肺腺癌 (胸水)
















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