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        293T 人胚腎細胞

        簡要描述:CRL-11268 293T 人胚腎細胞
        ATCC 細胞|細胞系|細胞株|腫瘤細胞|細胞|貼壁細胞|懸浮細胞|。細胞庫管理規(guī)范,提供的細胞株背景清楚,提供參考文獻和培養(yǎng)條件

        • 產(chǎn)品型號:CRL-11268
        • 廠商性質(zhì):生產(chǎn)廠家
        • 更新時間:2025-08-14
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        CRL-11268 293T 人胚腎細胞

        ATCC® Number:  CRL-11268™

        Designations:  293T/17 [HEK 293T/17]

        Depositors:   Rockefeller Univ.

        Biosafety Level: 2 [Cells contain Adeno and SV-40 viral DNA sequences ]

        Shipped:  frozen

        Medium & Serum:  See Propagation

        Growth Properties: adherent

        Organism: Homo sapiens (human)

        Morphology: epithelial

        CRL-11268 293T 人胚腎細胞

        Source: Organ: kidney

        Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


        Restrictions: The line is available with the following restriction: 1. The cell line was deposited at the ATCC by Rockefeller University and is provided for research purposes only. Neither the cell line nor the products derived from it may be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purposes of sale, or producing for sale, cells or their products. The cells are provided as a service to the research community. They are provided without warranty of merchantability or fitness for a particular purpose or any other warranty, expressed or implied. 2. Any proposed commercial use of the cells, or their products, must first be negotiated with Cell Genesys, 500 Forbes Boulevard, South San Francisco, CA 94080 Attn: Robert H. Tidwell; Senior Vice President, Corporate Development.

        Antigen Expression: SV40 T antigen [45408]

        Age:  fetus

        Comments: The 293T/17 cell line is a derivative of the 293T (293tsA1609neo) cell line. 293T is a highly transfectable derivative of the 293 cell line into which the temperature sensitive gene for SV40 T-antigen was inserted. 293T cells were cloned and the clones tested with the pBND and pZAP vectors to obtain a line capable of producing high titers of infectious retrovirus, 293T/17. These cells constitutively express the simian virus 40 (SV40) large T antigen, and clone 17 was selected specifically for its high transfectability.293T/17 cells were cotransfected with the pCRIPenv- and the pCRIPgag-2 vectors to obtain the ANJOU 65 (see ATCC CRL-11269) cell line.ANJOU 65 cells were cotransfected with the pCRIPgag-2 and pGPT2E vectors to obtain the BOSC 23 (see ATCC CRL-11270) ecotropic envelope-expression packaging cell line.ANJOU 65 cells were also cotransfected with the pCRIPAMgag vector along with a plasmid expressing the gpt resistance gene to obtain the Bing (see ATCC CRL-11554) amphotropic envelope-expression packaging cell line.

        Propagation:  ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

        Temperature: 37.0°C

        CRL-11268

        Subculturing:  Protocol:

        Remove and discard culture medium.

        Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

        Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

        Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

        Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

        Add appropriate aliquots of the cell suspension to new culture vessels.

        Incubate cultures at 37°C.

        Subc*tion Ratio: A subc*tion ratio of 1:4 to 1:8 is recommended

        Medium Renewal: Every 2 to 3 days

        Preservation:  Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

        Storage temperature: liquid nitrogen vapor phase

        Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

        recommended serum:ATCC 30-2020

        derivative:ATCC CRL-11269

        References: 45408: Sena-Esteves M, et al. Single-step conversion of cells to retrovirus vector producers with herpes simplex virus-Epstein-Barr virus hybrid amplicons. J. Virol. 73: 10426-10439, 1999. PubMed: 10559361

        57446: Pensiero M, et al. Retroviral vectors produced by producer cell lines resistant to lysis by human serum. US Patent 5,952,225 dated Sep 14 1999

        57447: Pensiero M, et al. Retroviral vectors produced by producer cell lines resistant to lysis by human serum. US Patent 6,329,199 dated Dec 11 2001

        57448: Pear WS, et al. Production of High-Titer Helper-Free Retroviruses by Transient Transfection. Proc. Natl. Acad. Sci. USA 90: 8392-8396, 1993. PubMed: 7690960




















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