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        當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞系>胃癌細(xì)胞系>NCI-N87 N87 人胃癌細(xì)胞

        NCI-N87 N87 人胃癌細(xì)胞

        簡要描述:NCI-N87 [N87] 人胃癌細(xì)胞
        貨號:NCI-N87 [N87]
        數(shù)量:大量
        生長狀態(tài):正常
        運(yùn)輸方式:常溫
        物種來源:人
        ATCC Number:CRL-5822
        細(xì)胞類型:普通細(xì)胞株-科研實(shí)驗(yàn)
        供應(yīng)商:復(fù)祥生物

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        • 更新時間:2025-07-08
        • 訪  問  量:2815

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        NCI-N87 [N87] 人胃癌細(xì)胞

        RPMI-1640(GIBCO)+10%胎牛血清。細(xì)胞貨期8-10個工作日

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        CRL-5822 NCI-N87 [N87] 人胃癌細(xì)胞

        ATCC® Number: CRL-5822™

        Designations: NCI-N87 [N87]

        Depositors: J Park

        Biosafety Level: 1

        Shipped: frozen

        Medium & Serum: See Propagation

        Growth Properties: adherent

        Organism: Homo sapiens (human)

        Morphology: epithelial


        Source: Organ: stomach

        Disease: gastric carcinoma

        Derived from metastatic site: liver

        Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


        Isolation: Isolation date: 1976

        Receptors: acetylcholine, muscarinic, expressed [23078]

        Tumorigenic: Yes

        Oncogene: myc +; erb B2 +

        DNA Profile (STR): Amelogenin: X,Y

        CSF1PO: 8,12

        D13S317: 8,11

        D16S539: 9,13

        D5S818: 12,13

        D7S820: 10,11

        THO1: 9

        TPOX: 9,11

        vWA: 15,16

        Cytogenetic Analysis: near diploid; DM were present in 64% of cells examined

        Gender: male

        Comments: NCI-N87 cells express the surface glycoproteins carcinoembryonic antigen (CEA) and TAG 72, and are L-dopa decarboxylase (DDC) negative. They were minimally positive for vasoactive intestinal peptide (VIP) receptors and lacked gastrin receptors. They were fiound to express receptors for muscarinic cholinergic agents. No evidence of amplification or rearrangements was noted with the N-myc, L-myc, myb and EGF receptor genes. The cell line expressed levels of c-myc and c-erb-B 2 RNA that were comparable to other cell lines.There was no expression of the following genes: N-myc, L-myc, c-cis, IGF-2, or gastrin releasing peptide. NCI-N87 cells have a reported plating efficiency of 4.3%.

        Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

        Atmosphere: air, 95%; carbon dioxide (CO2), 5%

        Temperature: 37.0°C

        Growth Conditions: They grow as an adherent monolayer of tightly knit epithelial cells.

        Subculturing: Protocol:

        Remove and discard culture medium.

        Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

        Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

        Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

        Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

        Add appropriate aliquots of the cell suspension to new culture vessels.

        Incubate cultures at 37°C.

        Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:4 is recommended

        Medium Renewal: Two to three times weekly

        Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

        Storage temperature: liquid nitrogen vapor phase

        Doubling Time: 47 hrs

        Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

        recommended serum:ATCC 30-2020

        References: 23078: Park JG, et al. Characteristics of cell lines established from human gastric carcinoma. Cancer Res. 50: 2773-2780, 1990. PubMed: 2158397

        23570: . NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996..

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