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        人外周血嗜堿性白細(xì)胞

        簡(jiǎn)要描述:CRL-2099 KU812 人外周血嗜堿性白細(xì)胞,
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        • 產(chǎn)品型號(hào):KU812
        • 廠商性質(zhì):生產(chǎn)廠家
        • 更新時(shí)間:2025-04-25
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        CRL-2099 KU812 人外周血嗜堿性白細(xì)胞

        ATCC® Number: CRL-2099™ Price: $329.00

        Designations: KU812

        Depositors: K Kishi

        Biosafety Level: 1

        Shipped: frozen

        Medium & Serum: See Propagation

        Growth Properties: suspension

        Organism: Homo sapiens (human)

        Morphology: myeloblast

        CRL-2099 KU812 人外周血嗜堿性白細(xì)胞

        Source: Organ: peripheral blood

        Disease: chronic myelogenous leukemia

        Cell Type: basophil;

        Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


        Isolation: Nigata Japan

        Isolation date: August 1, 1981

        Antigen Expression: CD3; Homo sapiens

        alanyl (membrane) aminopeptidase (ANPEP, CD13); Homo sapiens

        Cytogenetic Analysis: 58, XY, t(9;22)(q34;q11), i(17q), +1, +4, +6, +6, +8, +der(9)t(9;22), + der(9)t(9;22), +der(11)q(11), +14, +15, +19, +21, +21, -9 [44220]

        Age: 38 years adult

        Gender: male

        Ethnicity: Japanese

        Comments: The KU812 cell line was established from the peripheral blood of a patient in blast crisis of chronic myelogenous leukemia. The cells contain at least one Ph1 (Philadelphia) chromosome. The cell line has some characteristics of basophilic leukocytes (Fc receptors, basophilic granules, histamine production), and are negative for lymphoid markers.

        Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

        Atmosphere: air, 95%; carbon dioxide (CO2), 5%

        Temperature: 37.0°C

        Subculturing: Protocol: Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 3 X 10(5) viable cells/ml. . Do not allow the cell concentration to exceed 3 x 10(6) cells/ml.

        Medium Renewal: every 2 to 3 days

        Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

        Storage temperature: liquid nitrogen vapor phase

        Doubling Time: 20 to 30 hours

        Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

        recommended serum:ATCC 30-2020

        derivative:ATCC CRL-2100

        derivative:ATCC CRL-2101

        References: 44220: Kishi K. A new leukemia cell line with Philadelphia chromosome characterized as basophil precursors. Leuk. Res. 9: 381-390, 1985. PubMed: 3858609

        44221: Okano H, et al. Hemoglobin synthesis of both adult and fetal types in a human CML cell line. J. Biochem. 104: 162-164, 1988. PubMed: 2460439

        44222: Okamura S, et al. Expression of cytokine genes in hematological malignancies. Nippon Ketsueki Gakkai Zasshi 52: 1423-1432, 1989. PubMed: 2698048

        61145: Blom T, et al. Phenotypic characterization of KU812, a cell line identified as an immature human basophilic leukocyte. Eur. J. Immunol. 22: 2025-2032, 1992. PubMed: 1639103


        CRL-2099 KU812



















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