<label id="mboud"><meter id="mboud"></meter></label>

        <rt id="mboud"><small id="mboud"><strike id="mboud"></strike></small></rt>
        咨詢熱線

        15000266580

        當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>人腫瘤細(xì)胞、癌細(xì)胞>Panc 03.27 人胰腺癌細(xì)胞

        Panc 03.27 人胰腺癌細(xì)胞

        簡(jiǎn)要描述:CRL-2549 Panc 03.27
        人胰腺癌細(xì)胞,原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;
        細(xì)胞庫(kù)管理規(guī)范,
        提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件!
        Panc 03.27 人胰腺癌細(xì)胞

        • 產(chǎn)品型號(hào):
        • 廠商性質(zhì):生產(chǎn)廠家
        • 更新時(shí)間:2025-07-19
        • 訪  問(wèn)  量:2013

        產(chǎn)品分類

        Product Category

        詳細(xì)介紹

        CRL-2549 Panc 03.27 人胰腺癌細(xì)胞

        ATCC® Number: CRL-2549™ Price: $417.00

        Designations: Panc 03.27

        Depositors: EM Jaffee

        Biosafety Level: 1

        Shipped: frozen

        Medium & Serum: See Propagation

        Growth Properties: adherent

        Organism: Homo sapiens (human)

        Morphology: epithelial

        Source: Organ: pancreas

        Disease: adenocarcinoma

        Cellular Products: cytokeratins 7 and 18

        Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


        CRL-2549 Panc 03.27 人胰腺癌細(xì)胞

        Tumorigenic: Yes

        Oncogene: K-ras + (wild-type)

        Antigen Expression: MHC class I +; MHC class II - [50655]

        Blood type A; Rh+

        DNA Profile (STR): Amelogenin: X

        CSF1PO: 10,11

        D13S317: 11,12

        D16S539: 12

        D5S818: 12,13

        D7S820: 8,13

        THO1: 6

        TPOX: 8,9

        vWA: 16,17

        Age: 65 years

        Gender: female

        Ethnicity: White

        Comments: Panc 03.27 is a pancreatic adenocarcinoma epithelial cell line derived in 1995 from a primary tumor removed from the head-of-the-pancreas of a female with pancreatic adenocarcinoma.The cells have a reported plating efficiency of 80%.

        Propagation: ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate and supplemented with 10 Units/ml human insulin, 85%; fetal bovine serum, 15%

        Atmosphere: air, 95%; carbon dioxide (CO2), 5%

        Temperature: 37.0°C

        Subculturing: Protocol:

        1.Remove and discard culture medium.

        2.Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

        3.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

        Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.

        4.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

        5.Add appropriate aliquots of the cell suspension to new culture vessels.

        6.Incubate cultures at 37C.

        Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:4 is recommended

        Medium Renewal: Every 2 to 3 days

        Preservation: Freeze medium: culture medium, 95%; DMSO, 5%

        Storage temperature: liquid nitrogen vapor phase

        Doubling Time: 37.5 hrs

        Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

        recommended serum:ATCC 30-2020

        0.25% (w/v) Trypsin - 0.53 mM EDTA in Hank' BSS (w/o Ca++, Mg++):ATCC 30-2101

        Cell culture tested DMSO:ATCC 4-X

        References: 50655: Jaffee EM, et al. Development and characterization of a cytokine-secreting pancreatic adenocarcinoma vaccine from primary tumors for use in clinical trials. Cancer J. Sci. Am. 4: 194-203, 1998. PubMed: 9612602


        CRL-2549





















        產(chǎn)品咨詢

        留言框

        • 產(chǎn)品:

        • 您的單位:

        • 您的姓名:

        • 聯(lián)系電話:

        • 常用郵箱:

        • 省份:

        • 詳細(xì)地址:

        • 補(bǔ)充說(shuō)明:

        • 驗(yàn)證碼:

          請(qǐng)輸入計(jì)算結(jié)果(填寫(xiě)阿拉伯?dāng)?shù)字),如:三加四=7
        聯(lián)系方式

        郵箱:xiangfbio@163.com

        地址:上海市虹口區(qū)四平路710號(hào)7層

        咨詢熱線

        400-821-8510

        (周一至周日9:00- 19:00)

        在線咨詢
        • 掃一掃 微信咨詢

        Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
        技術(shù)支持:化工儀器網(wǎng)    管理登陸
        主站蜘蛛池模板: 中国好声音第二季免费播放| 亚洲国产精品白丝在线观看| 成人AV免费网址在线观看| 丁香花免费高清视频完整版| 久久青草亚洲AV无码麻豆| 亚洲午夜国产精品| 最新亚洲人成网站在线观看| 在线jyzzjyzz免费视频| 久久国产亚洲精品麻豆| 中中文字幕亚洲无线码| 黄瓜视频高清在线看免费下载| 亚洲av日韩av综合| 两个人看的www免费视频| 亚洲精品无码永久中文字幕| 中文字幕成人免费高清在线视频 | 一区二区三区免费视频观看| 亚洲精品高清在线| 中国极品美軳免费观看| 综合自拍亚洲综合图不卡区| 在线观看av永久免费| 大桥未久亚洲无av码在线| 亚洲国产精品成人| 亚洲另类图片另类电影| 在线a人片天堂免费观看高清| WWW亚洲色大成网络.COM| 国产成人亚洲综合无码| 午夜视频在线免费观看| 亚洲a视频在线观看| 国产色婷婷精品免费视频| 亚洲伊人精品综合在合线| 成人激情免费视频| 国产免费黄色无码视频| 亚洲一区二区三区四区在线观看| 青青草免费在线视频| 一级做a爰片久久毛片免费陪 | 亚洲午夜精品第一区二区8050| 国产羞羞的视频在线观看免费| 一本久久a久久精品亚洲| 在线观看永久免费| 免费看又黄又爽又猛的视频软件| 大香人蕉免费视频75|