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        SW-13 人腎上腺皮質(zhì)腺癌細(xì)胞

        簡要描述:CCL-105 SW-13 人腎上腺皮質(zhì)腺癌細(xì)胞,
        原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫管理規(guī)范,
        提供的細(xì)胞株背景清楚,提供參考文獻和培養(yǎng)條件!

        • 產(chǎn)品型號:CCL-105
        • 廠商性質(zhì):生產(chǎn)廠家
        • 更新時間:2025-05-23
        • 訪  問  量:1977

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        CCL-105 SW-13 人腎上腺皮質(zhì)腺癌細(xì)胞的介紹

        CCL-105 SW-13 人腎上腺皮質(zhì)腺癌細(xì)胞

        ATCC® Number: CCL-105™ Price: $323.00

        Designations: SW-13

        Depositors: A Leibovitz

        Biosafety Level: 1

        Shipped: frozen

        Medium & Serum: See Propagation

        Growth Properties: adherent

        Organism: Homo sapiens (human)

        Morphology: epithelial


        Source: Organ: adrenal gland

        Tissue: cortex

        Tumor Stage: grade IV

        Disease: primary small cell carcinoma

        Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


        Isolation: Isolation date: August, 1971

        Virus Susceptibility: Human poliovirus 1

        Vesicular stomatitis virus

        Reverse Transcript: negative

        DNA Profile (STR): Amelogenin: X

        CSF1PO: 11,12

        D13S317: 9

        D16S539: 12

        D5S818: 12

        D7S820: 8,10

        THO1: 7,8

        TPOX: 8

        vWA: 17,19

        Cytogenetic Analysis: modal number = 63; range = 45 to 65.

        Approximay 10% of the cells examined possessed a pair of dicentric chromosomes.

        Isoenzymes: G6PD, B

        Age: 55 years

        Gender: female

        Ethnicity: Caucasian

        Comments: Electron microscopic studies show many bulb gap junctions (BGJ).

        Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

        Temperature: 37.0°C

        Subculturing: Protocol:

        1.Remove and discard culture medium.

        2.Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

        3.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

        Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

        4.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

        5.Add appropriate aliquots of the cell suspension to new culture vessels.

        6.Incubate cultures at 37°C.

        Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:8 is recommended

        Medium Renewal: 2 to 3 times per week

        Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

        Storage temperature: liquid nitrogen vapor phase

        Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2008

        recommended serum:ATCC 30-2020

        References: 22140: . . In Vitro 8: 443, 1973.

        22526: Leibovitz A, et al. New human cancer cell culture lines. I. SW-13, small-cell carcinoma of the adrenal cortex. J. Natl. Cancer Inst. 51: 691-697, 1973. PubMed: 4765382

        22536: Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871

        22539: Fogh J, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080

        23212: Lasfargues EY, Ozzello L. C*tion of human breast carcinomas. J. Natl. Cancer Inst. 21: 1131-1147, 1958. PubMed: 13611537

        26278: Johnson RG, Sheridan JD. Junctions between cancer cells in culture: ultrastructure and permeability. Science 174: 717-719, 1971. PubMed: 4330805

        26279: Pinto da Silva P, Gilula NB. Gap junctions in normal and transformed fibroblasts in culture. Exp. Cell Res. 71: 393-401, 1972. PubMed: 4339896

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