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        TM3 小鼠gaowan間質細胞

        簡要描述:CRL-1714 TM3 小鼠gaowan間質細胞,原代細胞|細胞系|細胞株|菌種;細胞庫管理規范,提供的細胞株背景清楚,提供參考文獻和培養條件!

        • 產品型號:CRL-1714
        • 廠商性質:生產廠家
        • 更新時間:2025-07-19
        • 訪  問  量:2405

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        CRL-1714 TM3 小鼠gaowan間質細胞的詳細介紹

        CRL-1714 TM3 小鼠gaowan間質細胞

        ATCC® Number:CRL-1714™  Price:
        Designations:TM3

        Depositors:JP Mather

        Biosafety Level:1

        Shipped:frozen

        Medium & Serum:See Propagation

        Growth Properties:adherent

        Organism:Mus musculus (mouse)

        Morphology:epithelial


        Source:Organ: testis
        Disease: normal
        Cell Type: Leydig cell;


        Cellular Products:prostaglandin F2a

        Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
        CRL-1714

        Receptors:luteinizing hormone (LH); epidermal growth factor (EGF); androgen receptor, positive; estrogen receptor, positive; progesterone receptor, positive

        Tumorigenic:No

        Age:11 to 13 days

        Gender:male

        Comments:The TM3 cell line responds to LH with an increase in cAMP production, but does not respond to follicle stimulating hormone (FSH).

        The maintenance of responsiveness to LH is dependent upon serum lot.

        In the presence of LH, the cells are capable of metabolizing cholesterol.

        Tested and found negative for ectromelia virus (mousepox).



        Propagation:ATCC complete growth medium: A 1:1 mixture of Ham's F12 medium and Dulbecco's modified Eagle's medium with 2.5mM L-Glutamine, 0.5mM Sodium Pyruvate, 1.2 g/L Sodium Bicarbonate and 15 mM HEPES, 92.5%; horse serum, 5%; fetal bovine serum, 2.5%

        Subculturing:Protocol: Remove medium, add fresh 0.25% trypsin-0.53mM EDTA solution, rinse, remove trypsin and let the culture sit at room temperature for 2 to 4 minutes. Add fresh medium, aspirate and dispense into new flasks.
        Subc*tion Ratio: A subc*tion ratio of 1:20 to 1:200 is recommended
        Medium Renewal: Every 3 to 4 days


        Preservation:Freeze medium: Complete growth medium, 95%, DMSO, 5%
        Storage temperature: liquid nitrogen vapor phase


        References:1158: Mather JP. Establishment and characterization of two distinct mouse testicular epithelial cell lines. Biol. Reprod. 23: 243-252, 1980. PubMed: 6774781

        1159: Mather JP, et al. Culture of testicular cells in hormone-supplemented serum-free medium. Ann. N.Y. Acad. Sci. 383: 44-68, 1982. PubMed: 7046561




















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