<label id="mboud"><meter id="mboud"></meter></label>

        <rt id="mboud"><small id="mboud"><strike id="mboud"></strike></small></rt>
        咨詢熱線

        15000266580

        當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>大鼠正常細(xì)胞>CRL-1442 BRL 3A 大鼠肝細(xì)胞

        CRL-1442 BRL 3A 大鼠肝細(xì)胞

        簡要描述:CRL-1442 BRL 3A 大鼠肝細(xì)胞, ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件

        • 產(chǎn)品型號:
        • 廠商性質(zhì):生產(chǎn)廠家
        • 更新時(shí)間:2025-05-24
        • 訪  問  量:2241

        產(chǎn)品分類

        Product Category

        詳細(xì)介紹

        CRL-1442 BRL 3A 大鼠肝細(xì)胞, ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)i優(yōu)培養(yǎng)條件


        CRL-1442 BRL 3A 大鼠肝細(xì)胞 的詳細(xì)介紹


        ATCC® Number:  CRL-1442™

        Designations:  BRL 3A

        Depositors:   SP Nissley, MM Rechler

        Biosafety Level: 1

        Shipped:  frozen

        Medium & Serum:  See Propagation

        Growth Properties: adherent

        Organism: Rattus norvegicus (rat)

        Morphology:


        Source: Organ: liver

        Strain: Buffalo

        Cellular Products: somatomedin like multiplication stimulating activity (MSA)

        Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


        Comments: The serum-free conditioned medium from this cell line is a source of MSA. MSA is a family of polypeptides that can partially satisfy the serum reguirement of chick embryo fibroblasts. [1060]

        Propagation:  ATCC complete growth medium: Minimum essential medium (Eagle) with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate, 90%; fetal bovine serum, 10%

        Atmosphere: air, 95%; carbon dioxide (CO2), 5%

        Temperature: 37.0°C

        Growth Conditions: For 24 hrs after initiating culture from a thawed ampule or after subculturing, grow the cells in culture medium with 5% fetal bovine serum. After 24 hrs, the cells may be transferred to serum-free medium.

        Subculturing:  Protocol:

        Remove and discard culture medium.

        Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

        Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

        Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

        Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

        To remove trypsin-EDTA solution, transfer cell suspension to centrifuge tube and spin at approximay 125 xg for 5 to 10 minutes.Discard supernatant and resuspend cells in fresh growth medium. Add appropriate aliquots of cell suspension to new culture vessels.

        Incubate cultures at 37°C.




        Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:6 is recommended

        Medium Renewal: 2 times per week

        Preservation:  Freeze medium: Complete growth medium supplemented with 10% fetal bovine serum and 5% DMSO

        Storage temperature: liquid nitrogen vapor phase

        Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003

        References: 1060: Nissley SP, et al. Proliferation of buffalo rat liver cells in serum-free medium does not depend upon multiplication-stimulating activity (MSA). Cell 11: 441-446, 1977. PubMed: 302146

        22582: Coon HG, Weiss MC. A quantitative comparison of formation of spontaneous and virus- produced viable hybrids. Proc. Natl. Acad. Sci. USA 62: 852-859, 1969. PubMed: 4308097

        22711: Dulak NC, Temin HM. A partially purified polypeptide fraction from rat liver cell conditioned medium with multiplication-stimulating activity for embryo fibroblasts. J. Cell. Physiol. 81: 153-170, 1973. PubMed: 4735141

        22712: Dulak NC, Shing YW. Large scale purification and further characterization of a rat liver cell conditioned medium multiplication stimulating activity. J. Cell. Physiol. 90: 127-130, 1977. PubMed: 833209

        22762: Schalch DS, et al. Nonsuppressible insulin-like activity (NSILA). I. Development of a new sensitive competitive protein-binding assay for determination of serum levels. J. Clin. Endocrinol. Metab. 46: 664-671, 1978. PubMed: 755052























        產(chǎn)品咨詢

        留言框

        • 產(chǎn)品:

        • 您的單位:

        • 您的姓名:

        • 聯(lián)系電話:

        • 常用郵箱:

        • 省份:

        • 詳細(xì)地址:

        • 補(bǔ)充說明:

        • 驗(yàn)證碼:

          請輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
        聯(lián)系方式

        郵箱:xiangfbio@163.com

        地址:上海市虹口區(qū)四平路710號7層

        咨詢熱線

        400-821-8510

        (周一至周日9:00- 19:00)

        在線咨詢
        • 掃一掃 微信咨詢

        Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
        技術(shù)支持:化工儀器網(wǎng)    管理登陸
        主站蜘蛛池模板: 全免费a级毛片免费看不卡| 亚洲人成免费网站| 日韩特黄特色大片免费视频| 亚洲美女色在线欧洲美女| 精品福利一区二区三区免费视频| 亚洲最大福利视频网站| 亚洲毛片免费视频| 色天使亚洲综合在线观看| 欧美a级成人网站免费| 亚洲av永久无码一区二区三区| 午夜视频免费观看| 美女无遮挡免费视频网站| 免费一级特黄特色大片在线| 皇色在线免费视频| 亚洲第一福利视频| 西西大胆无码视频免费| 亚洲国产成人AV网站| 国产精品亚洲玖玖玖在线观看| 国产人成网在线播放VA免费| 麻豆亚洲AV永久无码精品久久| 国产免费AV片在线播放唯爱网 | 免费能直接在线观看黄的视频 | 国产成人va亚洲电影| 亚洲M码 欧洲S码SSS222| 中文字幕乱理片免费完整的| 久久久久亚洲AV无码网站| AV免费网址在线观看| 成人免费网站久久久| 亚洲av无码一区二区三区不卡| 1000部拍拍拍18免费网站| 亚洲s码欧洲m码吹潮| 日韩亚洲变态另类中文| 亚洲精品视频在线观看免费| 美国毛片亚洲社区在线观看| 亚洲人成在线播放网站| 日韩精品福利片午夜免费观着| 一级做a毛片免费视频| 亚洲国产成人久久| 亚洲人成人网站在线观看| aⅴ在线免费观看| 午夜在线免费视频|