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        RBL-1 大鼠嗜堿性粒細(xì)胞白血病細(xì)胞

        簡(jiǎn)要描述:CRL-1378 RBL-1 大鼠嗜堿性粒細(xì)胞白血病細(xì)胞, ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件

        • 產(chǎn)品型號(hào):CRL-1378
        • 廠商性質(zhì):生產(chǎn)廠家
        • 更新時(shí)間:2025-05-24
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        CRL-1378 RBL-1 大鼠嗜堿性粒細(xì)胞白血病細(xì)胞, ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)優(yōu)培養(yǎng)條件

        CRL-1378 RBL-1 大鼠嗜堿性粒細(xì)胞白血病細(xì)胞 的詳細(xì)介紹

        CRL-1378

        ATCC® Number:CRL-1378™  Price:$329.00
        Designations:RBL-1

        Depositors:H Metzger, C Isersky

        Biosafety Level:1

        Shipped:frozen

        Medium & Serum:See Propagation

        Growth Properties:suspension

        Organism:Rattus norvegicus (rat)

        Morphology:lymphoblast


        Source:Organ: peripheral blood
        Strain: Wistar
        Disease: leukemia
        Cell Type: basophil; chemically induced


        Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.



        Applications:transfection host (Nucleofection technology from Lonza)

        Receptors:FcERI (Fc of IgE)

        Comments:The line exhibits various characteristics of basophil differentiation including surface receptors for IgE. It was shown to not release histamine by an IgE mediated system. PubMed: 6166481

        Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
        Temperature: 37.0°C


        Subculturing:Protocol: Cultures can be maintained by the addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 viable cells/ml and maintain between 1 X 10 exp5 cells/ml and 1 X 10 exp6 /ml..
        Medium Renewal: Add medium as cell density increases


        Preservation:Freeze medium: Complete growth medium 95%; DMSO, 5%
        Storage temperature: liquid nitrogen vapor phase


        Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003

        recommended serum:ATCC 30-2020



        References:1232: Kulczycki A Jr., et al. The interaction of IgE with rat basophilic leukemia cells. I. Evidence for specific binding of IgE. J. Exp. Med. 139: 600-616, 1974. PubMed: 4812630

        22475: Barsumian EL, et al. IgE-induced histamine release from rat basophilic leukemia cell lines: isolation of releasing and nonreleasing clones. Eur. J. Immunol. 11: 317-323, 1981. PubMed: 6166481

        22638: Eccleston E, et al. Basophilic leukaemia in the albino rat and a demonstration of the basopoietin. Nat. New Biol. 244: 73-76, 1973.























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