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        CRL-2095 CAL 27 人舌鱗癌細(xì)胞

        簡要描述:CRL-2095 CAL 27 人舌鱗癌細(xì)胞,ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|細(xì)胞,細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件。

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        • 更新時(shí)間:2025-04-27
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        CRL-2095 CAL 27 人舌鱗癌細(xì)胞

        ATCC® Number:CRL-2095™Price:
        Designations:CAL 27

        Depositors:C Cardona

        Biosafety Level:1

        Shipped:frozen

        Medium & Serum:See Propagation

        Growth Properties:adherent

        Organism:Homo sapiens (human)

        Morphology:epithelial


        Source:Organ: tongue
        Disease: squamous cell carcinoma


        Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.



        Isolation:Isolation date: 1982

        Tumorigenic:Yes

        DNA Profile (STR):

        Amelogenin: X

        CSF1PO: 10,12

        D13S317: 10,11

        D16S539: 11,12

        D5S818: 11,12

        D7S820: 10

        THO1: 6,9.3

        TPOX: 8

        vWA: 14,17

        CRL-2095 CAL 27 人舌鱗癌細(xì)胞



        Cytogenetic Analysis:aneuploid; modal number = 43

        Age:56 years

        Gender:male

        Ethnicity:Caucasian

        Comments:

        CAL 27 cells were resistant to treatment with VDS (vindesine sulfate), CDP (cis-platinum) or ACTD (actinomycin D).

        A culture submitted to the ATCC in December 1993 was found to be contaminated with mycoplasma. Progeny were cured by a 21-day treatment with BM Cycline.



        Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
        Temperature: 37.0°C


        Subculturin


        Preservation:Freeze medium: Complete growth medium, 95%; DMSO, 5%
        Storage temperature: liquid nitrogen vapor temperature


        Doubling Time:35 hrs

        Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

        recommended serum:ATCC 30-2020



        References:

        23521: Gioanni J, et al. Two new human tumor cell lines derived from squamous cell carcinomas of the tongue: establishment, characterization and response to cytotoxic treatment. Eur. J. Cancer Clin. Oncol. 24: 1445-1455, 1988. PubMed: 3181269
























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